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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Interactions between Major Bioactive Polyphenols of Sugarcane Top: Effects on Human Neural Stem Cell Differentiation and Astrocytic Maturation
doi: 10.3390/ijms232315120
Figure Lengend Snippet: STEE and its major polyphenolic constituents stimulated the mitochondrial activity of hNSCs-derived immature astrocytes. ( A ) hNSCs were plated in a 96 well plate, induced differentiation into astrocytes, and pre-treated with STEE and its major constituents; 3CQA, 5CQA, 3FQA, or ISO, for the indicated times (24, 48, or 72 h). Cells were then further incubated with Rh123 for 20 min, and the fluorescence intensity generated by Rh123 was monitored at λex = 507 nm and λem = 529 nm. The percentages shown are the mean ± SEM (n = 3–6). One-way ANOVA with Dunnett’s post hoc test for the comparisons: * p < 0.05. ( B ) Cells were incubated with STEE and its constituents for 48 h, and PGC-1α mRNA ( PPARGC1 ) expression was measured. The relative values are the mean ± SEM (n = 3) compared with the control. One-way ANOVA with Dunnett’s post hoc test for the comparisons: * p < 0.05, **** p < 0.0001. A description of the number for the treatment groups is indicated in . ( C ) Phase-contrast microphotographs of hNSC-derived astrocytes cultured for 48 h in the absence or presence of STEE (50 µg/mL). High magnification views of boxed areas are shown below panels. Scale bar = 50 µm in panels and 20 μm in insets.
Article Snippet:
Techniques: Activity Assay, Derivative Assay, Incubation, Fluorescence, Generated, Expressing, Control, Cell Culture